Journal: Developmental cell
Article Title: AAGAB controls AP2 adaptor assembly in clathrin-mediated endocytosis
doi: 10.1016/j.devcel.2019.06.013
Figure Lengend Snippet: KEY RESOURCE TABLE
Article Snippet: Dr. Roy Parker N/A Recombinant DNA Plasmid: pET-SUMO-humanAAGAB This paper N/A Plasmid: pGEX4T-3-mouseAP2A2 This paper N/A Plasmid: pACYC-Duet-ratAP2S1 This paper N/A Plasmid: p3×FLAG-humanAAGAB This paper N/A Plasmid: pcDNA3.1-mouseAP2A2-HA This paper N/A Plasmid: pcDNA3.1-ratAP2S1-HA This paper N/A Plasmid: pcDNA3.1-humanAP2B1-HA This paper N/A Plasmid: pcDNA3.1-mouseAP2M1-HA This paper N/A Plasmid: pcDNA3.1-mouseAP2A2 This paper N/A Plasmid: pCMV-ratAP2S1-3×FLAG This paper N/A Plasmid: pEGFP-N1-humanAAGAB This paper N/A Plasmid: pCSII-humanAAGAB This paper N/A Plasmid: p3×FLAG-CMV™-14 Sigma-Aldrich Cat# E7908 Plasmid: p3×FLAG-CMV™-7.1 Sigma-Aldrich Cat# E7533 Plasmid: pcDNA™3.1 (+) Thermo Fisher Scientific Cat# V79020 Plasmid: pLenti-HA-GLUT4-GFP (Muretta et al., 2008) N/A Plasmid: pLentiCRISPRv2 ( Sanjana et al., 2014 ) Addgene: 52961 Plasmid: pLenti-Guide-Hygro ( Gulbranson et al., 2017 ) N/A Plasmid: pLenti-CRISPR ( Shalem et al., 2014 ) Addgene: 49535 Plasmid: pAdVAntage Promega Cat# E1711 Plasmid: pCMV-VSVG ( Stewart et al., 2003 ) Addgene: 8454 Plasmid: psPAX2 Dr. Diderio Trono Addgene: 12260 Plasmid: pLenti-Guide-Puro ( Sanjana et al., 2014 ) Addgene: 52963 Plasmid: pAP1G1 (1-595)-GST ( Ren et al., 2013 ) Plasmid: pmCherry-α-adaptin Dr. Juan Bonifacino Software and Algorithms FASTX-toolkit Hannon lab website http://hannonlab.cshl.edu/fastx_toolkit/ MAGeCK ( Li et al., 2014 ) https://sourceforge.net/p/mageck/wiki/Home/ EXCEL Microsoft N/A KaleidaGraph Synergy Software http://www.synergy.com/wordpress_650164087/ Prism (version 7) GraphPad https://www.graphpad.com/scientific-software/prism/ R studio R-project N/A FlowJo (version 8 and 9) FlowJo N/A Open in a separate window KEY RESOURCE TABLE AAGAB binds to the AP2 complex and is essential for clathrin-mediated endocytosis AAGAB controls the sequential assembly of the AP2 adaptor complex AAGAB stabilizes AP2 complex intermediates A skin disease-causing mutation impairs the function of AAGAB The AP2 complex is required for clathrin-mediated endocytosis.
Techniques: Recombinant, Electron Microscopy, Protease Inhibitor, DNA Extraction, Mutagenesis, Sequencing, Amplification, CRISPR, Plasmid Preparation, Software
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